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Biochemistry
Western blot
Bubble

In WB, after electrophoresis transfer, why there are too many bubbles in the membrane?

JC
Jeongpil Cheng Apr 26, 2022

Just as what I described in the question title. Could you please give me some advice?


Thanks a lot, guys~
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Hailemariam Gutema Answered Apr 27, 2022

Institute of Cytology of Russian Academy of Sciences

Usually, it is because you failed to squeeze the bubbles between the membrane and the gel. Use new filter paper that is good quality. Just wear a pair of clean rubber gloves to squeeze the bubbles between the membrane and gel. Sweep it with great patience and care.

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