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DNA extraction

MS Maximilian R Stammnitz

Published: Mar 2, 2021 Views: 1216

Original research article

The authors used this protocol in:

https://doi.org/10.7554/eLife.61504

Jan 19, 2021

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Dear Kimberly,

 

Many thanks for getting in touch – raw DNA extraction yields from DNeasy PowerWater can be fairly low. In our case, most outputs ranged at ~900 ng total. You can obviously play with the kits descriptions a bit, e.g. extending beat beating, using higher input water volumes, etc. But even a few ng (and low 230 purity) would yield you plenty of DNA to amplify 16S from and sequence these with MinION!

 

All the best,
Max

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