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DNA Extraction from Dried Plant Tissues Using 96-well Format (cTab Method)
Published: Jul 5, 2011 DOI: 10.21769/BioProtoc.89 Views: 18191
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Abstract
This high throughput DNA isolation protocol is used to extract DNA of high quality from plant tissues for various genetics studies, like genotyping, and mapping etc. This protocol uses the well-established CTAB extraction procedure, and has been adapted to be used with 96-well plates.
Materials and Reagents
- Hexadecyltrimethyl Ammonium Bromide (CTAB) (Thermo Fisher Scientific)
- Sodium bisulfite
- Tungsten carbide beads
- Sodium chloride
- EDTA
- Tris-HCl (pH 8.0)
- β-mercapto-ethanol (BME)
- Chloroform
- Octanol
- Isopropanol
- Ethanol
- Sodium-acetate
- Ammonium-acetate
- TE (pH 8.0)
- 100 ml of CTAB (see Recipes)
Equipment
- Centrifuges (Eppendorf)
- Mixer mill
- Glass beaker
- Water bath
- 96-square well blocks
Procedure
Category
Molecular Biology > DNA > DNA extraction
Plant Science > Plant molecular biology > DNA
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