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Arabidopsis Pollen Tube Aniline Blue Staining
Published: Jun 20, 2011 DOI: 10.21769/BioProtoc.88 Views: 26754
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Abstract
The aim of this experiment is to track pollen tube growth in vivo in the female tissues after pollination. This can be used to phenotype pollen germination, tube growth and guidance, and reception.
Background
Materials and Reagents
- Stock solutions:
- Acetic acid
- Ethanol
- NaOH
- K2HPO4
- KH2PO4
- Aniline blue (Thermo Fisher Scientific)
- Glycerol
- Working solutions:
- 10% acetic acid in EtOH (fixative)
- 0.01% aniline blue in KPO4 buffer (dye)
- KPO4 buffer made with 50% glycerol (mounting media)
- KPO4 buffer (see Recipes)
Equipment
- Microscope with UV light
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Cell Biology > Tissue analysis > Tissue staining
Plant Science > Plant cell biology > Tissue analysis
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