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DAPI Nuclear Staining of Live Worm

Fanglian He Fanglian He

Published: Jun 5, 2011 DOI: 10.21769/BioProtoc.77 Views: 19086

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Abstract

Adapted from the Villenueve Lab at Stanford University. This is a very simple method using ethanol fixation, but works very well.

Background

Materials and Reagents

  1. Vectashield Mounting Media (Vector Labs)
  2. M9 solution
  3. 95% ethanol
  4. DAPI solution

Equipment

  1. Fluorescence microscope (Leica)
  2. Whatman paper
  3. Coverslip
  4. Nail polish

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Cell Biology > Cell staining > Nucleic acid

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