Pollen Fertility/viability Assay Using FDA Staining

Materials and Reagents

1. Fluorescein diacetate (FDA)
2. Excision: At the time of anthesis
3. Stain: Stock solution of FDA 2 mg FDA/ml of acetone (stored at -20 °C in an Eppendorf tube)
4. BK buffer S15 MOPS (see Recipes)
5. BK buffer S15 (see Recipes)
   

Equipment

1. Fluorescence microscope
2. Eppendorf tube
   

Procedure