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Pollen Fertility/viability Assay Using FDA Staining
Published: May 20, 2011 DOI: 10.21769/BioProtoc.75 Views: 30386
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Abstract
Pollen grains can be fertile or sterile by nature. This method stains pollen grains for an enzyme as the vital indicator of membrane integrity. Only fertile grains fluoresce under microscopic examination.
Keywords: Pollen
Fertility
Vital staining
Fluorescein diacetate
Pollen grain
Materials and Reagents
- Fluorescein diacetate (FDA)
- Excision: At the time of anthesis
- Stain: Stock solution of FDA 2 mg FDA/ml of acetone (stored at -20 °C in an Eppendorf tube)
- BK buffer S15 MOPS (see Recipes)
- BK buffer S15 (see Recipes)
Equipment
- Fluorescence microscope
- Eppendorf tube
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Cell Biology > Tissue analysis > Tissue staining
Plant Science > Plant physiology > Tissue analysis
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