Generation of TILs

The detailed TIL generation protocol is from reference 50, 51 as described in the supplementary data in the article.

For original articles please see:

(50)  M. E. Dudley, J. R. Wunderlich, T. E. Shelton, J. Even, S. A. Rosenberg, Generation of tumor-infiltrating lymphocyte cultures for use in adoptive             transfer therapy for melanomapatients. J. Immunother. 26, 332–342 (2003).PMID: 12843795

(51)    J. Jin, M. Sabatino, R. Somerville, J. R. Wilson, M. E. Dudley, D. F. Stroncek, S. A. Rosenberg, Simplified method of the growth of human tumor           infiltrating lymphocytes in gas-permeable flasks to numbers needed for patient treatment. J. Immunother. 35, 283–292 (2012).