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Single Worm PCR

Fanglian He Fanglian He

Published: Apr 20, 2011 DOI: 10.21769/BioProtoc.60 Views: 20849

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Abstract

This protocol is used for genotyping single worms.

Materials and Reagents

  1. Nonidet P-40 (Sigma-Aldrich, catalog number: 74385 )
  2. Gelatin (Sigma-Aldrich)
  3. Proteinase K (Promega Corporation, catalog number: V3021 )
  4. KCl
  5. Tris (pH 8.3)
  6. MgCl2
  7. Nonidet P-40
  8. Tween-20
  9. Gelatinin
  10. Lysis buffer (see Recipes)

Equipment

  1. PCR tubes

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Molecular Biology > DNA > PCR

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1 Q&A

I saw another protocol which said to use 0.45% Tween-20 instead...
edit 1 Answer 4 Views Nov 17, 2014