Cell cycle prediction

Cell cycle prediction analysis was performed using the Seurat software in R. 

We referenced this vignette to do our analysis:

https://satijalab.org/seurat/v3.1/cell_cycle_vignette.html

Under the heading: "Assign Cell-cycle Scores"

Reference to the code used to perform analysis and make figures was uploaded to gitlab and has been provided in our paper. The only modifcation made to the above vignette was removing genes in Seurat's cell cycle modules that we did not have in our dataset and using the remaining genes to run the command "CellCycleScoring":

https://gitlab.com/akakebee/kakebeen-et-al-2020/-/blob/master/SingleCell_1/UMAP/SCRNA_Seurat_UMAP_1.Rmd

Under the heading: "## Cell cycle analysis”