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Lifespan Assay

Fanglian He Fanglian He

Published: Apr 5, 2011 DOI: 10.21769/BioProtoc.57 Views: 27499

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Abstract

This assay is used to address aging-related questions in worms.

Materials and Reagents

  1. NGM medium
  2. 5-fluorodeoxyuridine (FUDR) (Sigma-Aldrich, catalog number: F0503 )

Equipment

  1. Incubators
  2. NGM agar plates (35 x 10 mm)
  3. 60 x 15 and 35 x 10 mm petri dish (VWR)

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Developmental Biology > Cell growth and fate

Cell Biology > Cell viability > Cell proliferation

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3 Q&A

So any idea of life span measuring without FuDR?
edit 0 Answer 1 View Jun 4, 2013

Per request of Dr. Fanglian He, I will continue to add more...
edit 0 Answer 4 Views Jul 8, 2012

how we can add the drug or plant extract to the NGM plates?
edit 1 Answer 3 Views Apr 18, 2012