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Yeast spot tests
Published: Aug 16, 2018 Views: 3586
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Grow strains overnight in YPD (5 ml is plenty)
Take plates out of cold room to warm up overnight
Pellet (2 min, 2000 rpm) and discard supernatant
Wash pellet in water
Pellet again and discard supernatant
Resuspend in 5 ml of water
Measure OD/ml
Dilute strains to 0.5 OD/ml in water
Make serial dilutions (1:10) – 4 or 5 is usually enough
Plate 4-5 µl of each dilution on each plate using multichannel pipette
Replicate plates are a good idea
Make sure spots don’t bleed into each other
Leave to dry before inverting plates
Place at 30C and check every day.
Take pictures on GelDoc (it has a plates setting)
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