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Chemicals and synthetic DNA

MR Monica Rosas-Lemus

Published: Oct 12, 2020 Views: 801

Original research article

The authors used this protocol in:

https://doi.org/10.1126/scisignal.abe1202

Sep 28, 2020

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Hello Janina,

 

The manual edits are just a second inspection after codon optimization (Gensmart) to identify and correct possible artificial ribosome binding sites produced by the software. You can request our plasmids from BEI. Also, please find bellow the optimized sequences used for this study. 

 

Hope this is helpful.

 

All the best,

Monica

 

 

NSP10 sequence+ Start and Stop codons

ATGGCGGGAAACGCAACTGAGGTACCCGCTAATTCGACCGTGCTGAGCTTCTGCGCGTTTGCAGTTGATGCGGCTAAAGCGTATAAGGACTACTTGGCTTCCGGTGGTCAGCCGATCACGAACTGCGTTAAAATGTTGTGCACGCACACCGGCACCGGCCAGGCAATTACCGTTACCCCGGAGGCGAACATGGATCAAGAGAGCTTTGGTGGCGCGAGCTGCTGCCTGTACTGCCGCTGCCATATTGACCACCCGAATCCAAAAGGCTTCTGCGACCTGAAGGGCAAA

TACGTGCAAATCCCGACCACCTGTGCCAATGATCCGGTTGGTTTCACCCTGAAGAACACCGTGTGCACTGTCTGTGGTATG TGG AAA GGC TATGG

TTGTTCTTGTGACCAACTGCGTGAACCGATGTTACAGTAA


 

 

Nsp16 sequence+ Start and Stop codons

ATGTCAAGTCAGGCATGGCAACCCGGAGTAGCTATGCCGAATCTGTACAAGATGCAGCGTATGCTGTTGGAAAAATGTGATCTGCAAAACTACGGCGA

CTCTGCTACTCTCCCAAAGGGCATTATGATGAACGTCGCCAAATACACCCAGCTTTGCCAATATCTGAACACCTTGACCCTCGCAGTGCCGTACA

ACATGCGTGTTATCCACTTTGGTGCGGGTAGCGACAAAGGCGTTGCTCCGGGTACGGCTGTGTTGCGCCAGTGGCTGCCGACCGGTACATTGCTG

GTGGATTCTGACCTGAATGATTTTGTCAGCGACGCAGATTCCACCTTAATCGGCGACTGTGCGACCGTTCACACCGCGAATAAATGGGATTTGAT

TATCAGCGACATGTATGATCCGAAGACCAAGAACGTGACGAAAGAGAACGACTCC AAA GAG GGCTTCTTCACCTATATCTGCGGTTTTATCCAGC

AGAAACTGGCACTGGGCGGCTCCGTGGCGATTAAAATCACCGAACATTCTTGGAATGCGGATCTGTACAAACTTATGGGTCATTTCGCATGGTGG

ACCGCGTTCGTTACCAATGTGAACGCCAGCAGCTCAGAAGCTTTCCTGATCGGGTGCAATTACCTGGGTAAACCGCGTGAGCAGATCGATGGTTA

TGTTATGCACGCGAACTACATTTTTTGGCGTAATACGAACCCGATTCAACTGTCGAGCTATAGCTTGTTTGATATGAGCAAGTTCCCGCTGAAGC

TGCGTGGTACTGCGGTAATGTCC CTT AAA GAG GGC CAAATTAACGACATGATTCTGAGCTTGCTGAGTAAGGGCCGTCTGATCATCCGCGAAAAC

AACCGCGTTGTTATTAGCTCGGACGTCCTGGTTAATAACTAA

 



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