Lifespan assay

Hi There,

we have a JoVe article on how we run the assay, which explains everything in detail (see below). We made it free to access so you should have no trouble. The only thing missing is that for the eLife paper we killed the bacteria by gamma irradiation to avoid minocycline affecting lifespan through killing bacteria. How long you have to irradiate the bacteria to kill them depends on your irradiation source. If you have an X-ray machine it is about 2h, if you have caesium source it is somewhere between 10 to 20h dependnet on the age of the source. Any other way killing the bacteria (sodium azide, quadruple antibiotics, UV, heat, etc.) did not reliably kill large amounts of bacteria in a way that is reliable.

Atricle

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197298/

cheers Michael