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MTT Assay of Cell Numbers after Drug/Toxin Treatment
Published: Apr 5, 2011 DOI: 10.21769/BioProtoc.51 Views: 25587
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Abstract
MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since these types of materials are expected to stimulate or inhibit cell viability and growth. Here, a general protocol is described to carry out an MTT assay on different types of cells.
Keywords: MTT assay
Cell numbers
Drug
Background
Materials and Reagents
- Raw264.7, MCF-7 or Hela cells
- Thiazolyl blue tetrazolium bromide (MTT) (Sigma-Aldrich, catalog number: M5655 )
- DMSO
- DPBS (Life Technologies, InvitrogenTM, catalog number: 14190-250 )
- General chemicals (Sigma-Aldrich)
Equipment
- 96 well plate
- Shaking table
- Paper towels
- Incubator
Procedure
Category
Cancer Biology > General technique > Cell biology assays > Cell viability
Cell Biology > Cell viability > Cell death
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