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MTT Assay of Cell Numbers after Drug/Toxin Treatment

RC Ran Chen

Published: Apr 5, 2011 DOI: 10.21769/BioProtoc.51 Views: 25503

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Abstract

MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since these types of materials are expected to stimulate or inhibit cell viability and growth. Here, a general protocol is described to carry out an MTT assay on different types of cells.

Keywords: MTT assay search Cell numbers search Drug search

Materials and Reagents

  1. Raw264.7, MCF-7 or Hela cells
  2. Thiazolyl blue tetrazolium bromide (MTT) (Sigma-Aldrich, catalog number: M5655 )
  3. DMSO
  4. DPBS (Life Technologies, InvitrogenTM, catalog number: 14190-250 )
  5. General chemicals (Sigma-Aldrich)

Equipment

  1. 96 well plate
  2. Shaking table
  3. Paper towels
  4. Incubator

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Cancer Biology > General technique > Cell biology assays

Cell Biology > Cell viability > Cell death

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1 Q&A

How many number of cells should be placed in each well for effective...
edit 1 Answer 5 Views Jul 20, 2011