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Total RNA Extraction from C. elegans

Fanglian He Fanglian He

Published: Mar 20, 2011 DOI: 10.21769/BioProtoc.47 Views: 30339

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Abstract

This protocol describes total RNA extraction from worms with or without using commercial RNA extraction kits.

Materials and Reagents

  1. C. elegans
  2. Trizol (Life Technologies, Gibco®, catalog number: 15596-026 )
  3. DEPC treated H2O (Life Technologies, Ambion®)
  4. Turbo DNase (Life Technologies, Ambion®, catalog number: AM2238 )
  5. RNeasy Mini kit (Life Technologies, Gibco®, catalog number: 15596-026 )
  6. Ethanol
  7. Chloroform
  8. Isopropanol
  9. Liquid nitrogen
  10. RNase-free EDTA
  11. KH2PO4
  12. Na2HPO4
  13. NaCl
  14. MgSO4
  15. M9 buffer (see Recipes)

Equipment

  1. 15-ml Corning tubes (Corning)
  2. RNase-free eppendorf tubes (Eppendorf)
  3. Filter tips (Eppendorf)
  4. Dissecting microscope
  5. Water bath

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Molecular Biology > RNA > RNA extraction

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1 Q&A

如果是秀麗隱桿線蟲，請問每管要幾隻線蟲才夠提取RNA？
edit 2 Answers 8 Views Mar 3, 2014