Silver Staining

Materials and Reagents

1. 99.5 % ethanol (Sigma-Aldrich )
2. Silver nitrate (AgNO3) (Sigma-Aldrich )
3. Formaldehyde solution (37 %) (Sigma-Aldrich)
4. Sodium carbonate (Sigma-Aldrich)
5. Sodium thiosulphate (Sigma-Aldrich)
6. EDTA (Sigma-Aldrich)
7. Sodium dithionite (Sigma-Aldrich)
8. 30% ethanol
9. Acetic acid
10. Stop solution (see Recipes)
11. Fixation solution (see Recipes)
12. Sensitizer solution (see Recipes)
13. Staining solution (see Recipes)
14. Developer solution (see Recipes)
    

Equipment

1. Shaker

Procedure