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Site Density Protocol
Published: Jul 5, 2012 DOI: 10.21769/BioProtoc.234 Views: 8658
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Abstract
The site densities of cell surface molecules are useful information for cell function analysis. Using antibody staining and commercial available calibration beads, this assay quantitatively determines the T cell receptor site density at the single T cell level. This method can be easily extended to quantify other surface molecule densities on different cells or beads.
Materials and Reagents
- PE-conjugated anti-mouse TCR Va2 monoclonal antibody B20.1 (BD)
- PE Rat IgG2a, λ Isotype Control (BD)
- QuantiBRITE PE beads tube (BD)
Equipment
- Countertop Centrifuge
- BD LSR flow
Procedure
Copyright: © 2012 The Authors; exclusive licensee Bio-protocol LLC.
Category
Immunology > Immune cell function > Lymphocyte
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