Oil Red O Staining of Fixed Worm

Materials and Reagents

1. 20% paraformaldehyde
   
2. Na₂EGTA
   
3. Spermidine 3 HCl (1 g) (Sigma-Aldrich, catalog number: 85578 )
   
4. Spermine (5 g) (Sigma-Aldrich, catalog number: 85590 )
   
5. Oil Red O (Sigma-Aldrich, catalog number: O0625 )
   
6. NaPIPES (pH 7.4)
   
7. Beta-ME
   
8. Isopropanol
   
9. DTT
   
10. Tris Base
    
11. HCl
    
12. PBS
    
13. Agarose
    
14. NaCl
    
15. KCl
    
16. Na₂HPO₄•7H₂O
    
17. KH₂PO₄
    
18. NaOH
    
19. 10x PBS buffer (see Recipes)
    
20. 1 M Tris-Cl (pH 7.4) (see Recipes)
    
21. 2x MRWB (see Recipes)
    
22. Reduction buffer (see Recipes)
    

Equipment

1. Eppendorf Thermomixer Shaker (Eppendorf, catalog number: EF4283A )
   
2. Dissecting stereo microscope (LEICA MZ12 )
   
3. Compound microscope (Nikon ECLIPSE, model: E600 )
   
4. Tabletop centrifuge
   
5. 15 ml conical tube
   
6. 1.5-ml Eppendorf tube
   
7. 0.2 µm filter
   

Procedure