ChIP Assay for Cell Culture

Materials and Reagents

1. Salmon Sperm DNA (Life Technologies, Invitrogen^TM, catalog number: AM9680 )
   
2. Protein inhibitors
   1. Phenylmethylsulfonyl fluoride (PMSF) (Sigma-Aldrich, catalog number: 78830-5G )
      
   2. Aprotinin (Sigma-Aldrich, catalog number: A3428-10MG )
      
   3. Pepstatin A (Sigma-Aldrich, catalog number: P5318-5MG )
      
3. Formaldehyde (Thermo Fisher Scientific, catalog number: F75F-1GAL )
   
4. Protein A agarose beads (Upstate, Millipore, catalog number: 16-157 )
   
5. General chemicals (Sigma-Aldrich)
   
6. DPBS (Life Technologies, Invitrogen^TM, catalog number: 14190-250 )
   
7. QIAGEN PCR purification kit (QIAGEN, catalog number: 28104 )
   
8. ChIP lysis buffer (see Recipes)
   
9. Low salt wash buffer (see Recipes)
   
10. High salt wash buffer (see Recipes)
    
11. LiCl buffer (see Recipes)
    
12. TE buffer (see Recipes)
    
13. Elution buffer (see Recipes)
    

Equipment

1. Standard bench-top refrigerated centrifuge that can reach at least 13,000 rpm
   
2. Conical tube
   
3. Rotating platform
   

Procedure