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Murine xenograft model
Published: Jun 5, 2012 DOI: 10.21769/BioProtoc.221 Views: 22047
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Abstract
This experiment is used to test the efficacies of chemo treatments or gene therapy in an in vivo system. In this protocol, the mouse xenograft model is used.
Background
Materials and Reagents
- Severe combined immunodeficiency (SCID) mice
- NaCl
- Pluronic acid
- Phosphate buffered saline (PBS)
- HBSS solution
Equipment
- 1 ml syringe with 22-24 gauge of needls
- Bioluminescent imaging instrument (in university core facility)
Procedure
Copyright: © 2012 The Authors; exclusive licensee Bio-protocol LLC.
Category
Cancer Biology > General technique > Animal models > Cell invasion
Cancer Biology > General technique > Drug discovery and analysis > Cell viability
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