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Injecting Zebrafish Embryos

LJ Lili Jing

Published: Jan 20, 2011 DOI: 10.21769/BioProtoc.22 Views: 19455

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Abstract

Zebrafish is convenient for transient genetic manipulations such as morpholino-mediated gene knockdown, DNA/mRNA-based genetic overexpression. These genetic approaches do not involve the challenge of making transgenic animals, and their effect can last up to a week. This protocol describes the detailed steps of injecting to zebrafish embryos.

Materials and Reagents

  1. Zebrafish
  2. Oil
  3. Zebrafish mating boxes (Aquatics)
  4. Mineral oil (Sigma-Aldrich, catalog number: M5904 )

Equipment

  1. Bench
  2. Forceps
  3. Injection
  4. Micrometer slide
  5. Micropipette puller (Sutter instrument)
  6. Microinjection system Pico-injector (Harvard Apparatus)
  7. Benchtop microscope (Leica Microsystems)
  8. Micrometer slide (3B Scientific)
  9. Glass capillary tubing (Thermo Fisher Scientific)
  10. Benchtop microcentrifuge (Biotool)

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Molecular Biology > DNA > Transformation

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1 Q&A

请问E3是什么试剂？可以保证胚胎不悬浮不脱水。
edit 1 Answer 1 View Oct 17, 2012