Chromatin Immunoprecipitation (ChIP) Protocol from Tissue

Materials and Reagents

1. Frozen tissue
   
2. Protease inhibitors complete mini pill (F. Hoffmann-La Roche)
   
3. 1 kb DNA ladder (Life Technologies, Invitrogen™)
   
4. Protein A- or protein G- agarose (Santa Cruz Biotechnology)
   
5. Phenol-chloroform-isoamylalchohol (Life Technologies, Invitrogen™)
   
6. Pellet paint (Novagen)
   
7. General chemicals (Sigma-Aldrich)
   
8. PBS
   
9. Hepes
   
10. Tris-HCl (pH 8.0)
    
11. EDTA
    
12. SDS
    
13. EGTA
    
14. NaCl
    
15. Triton X-100
    
16. Sodium deoxycholate
    
17. Agarose gel
    
18. HEPES-NaOH buffer (see Recipes)
    
19. ChIP lysis buffer (see Recipes)
    
20. RIPA buffer (see Recipes)
    
21. Digesting buffer (see Recipes)
    

Equipment

1. Centrifuges
2. Sonicator
3. Razor blade
4. Shaker
5. PCR machine

Procedure