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Monocyte isolation, macrophage differentiation, and OC generation
Published: Dec 28, 2022 Views: 690
Original research article
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Jun 29, 2022
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Thank you for your question. For macrophage differentiation medium, we used MEM alpha supplemented with 10% FBS and 100 U/mL and 100 mg/mL Pen Strep plus 50 ng/mL hMCSF. For OC differentiation medium, we used MEM alpha supplemented with 10% FBS and 100 U/mL and 100 mg/mL Pen Strep plus 20 ng/mL hMCSF and 50 ng/mL hRANKL.
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