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Protein Extraction from Mice Xenograft Tumor

HP Huan Pang

Published: Apr 5, 2012 DOI: 10.21769/BioProtoc.202 Views: 24189

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Abstract

Proteins from frozen tissue such as xenograft tumor need to be extracted efficiently without being degraded to make the best use of a limited resource and to ensure that an accurate representation of proteins in selected tumor tissue can be obtained. This protocol describes how to extract proteins from xenograft tumors in mice.

Background

Materials and Reagents

  1. NaCl
  2. Tris
  3. EDTA
  4. NP-40
  5. EGTA
  6. NaF
  7. Beta-glycerophosphate
  8. Sodium pyrophosphate
  9. Sodium vanadate
  10. Isoflurane
  11. PMSF
  12. Laemmli buffer
  13. Liquid nitrogen
  14. Phosphatase inhibitor 1/2 (Sigma-Aldrich, catalog number: P2850 , P5726 )
  15. BCA kit (Thermo Fisher Scientific, catalog number: 23227 )
  16. Lysis buffer(see Recipes)

Equipment

  1. Centrifuges
  2. Sonicator
  3. Western blotting equipment

Procedure

Copyright: © 2012 The Authors; exclusive licensee Bio-protocol LLC.

Category

Cancer Biology > General technique > Biochemical assays > Protein analysis

Biochemistry > Protein > Isolation and purification

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1 Q&A

Hello Dr. Pang. I was wondering if your protocol for protein...
edit 0 Answer 7 Views Jul 17, 2018