Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Peer-reviewed
Flourescent Immunostaining Protocol for a-Bungorotoxin (AChRs) in Zebrafish
Published: Feb 20, 2012 DOI: 10.21769/BioProtoc.183 Views: 13126
PDF 
Ask a question
How to
cite
Favorite
Cited by
Abstract
Zebrafish embryo is convenient for studying neuromuscular system due to its transparency. This protocol is a rapid method to visualize the AChR clusters on the postsynaptic muscle membrane.
Background
Materials and Reagents
- Paraformaldehyde (USB Corporation, catalog number: 19943 )
- Phosphate buffered saline (PBS)
- DMSO
- Triton-100
- Na2HPO4
- NaH2PO4
- Collagenase (Sigma-Aldrich, catalog number: C-9891 )
- α-Bungorotoxin conjugated to Alexa 594 (Life Technologies, Invitrogen™, catalog number: B-13423)
- Vectashied (Vector Lab, catalog number: H-1400 )
- Primary antibody
- Secondary antibody
- Incubation Buffer (IB) (see Recipes)
- 0.1 M phosphate buffer(7.4) (see Recipes)
Equipment
- Rotator (Storvall Life Science, the Belly Dancer)
- Fluorescence microscope
- Transfer pipette
Procedure
Copyright: © 2012 The Authors; exclusive licensee Bio-protocol LLC.
Category
Neuroscience > Development > Immunofluorescence
Biochemistry > Protein > Immunodetection > Immunostaining
Cell Biology > Cell imaging > Fluorescence
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
