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Glucose Tolerance Test in Mice
Published: Oct 5, 2011 DOI: 10.21769/BioProtoc.159 Views: 63701
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Abstract
Glucose tolerance test is a standard procedure that addresses how quickly exogenous glucose can be cleared from blood. Specifically, uptake of glucose from the blood by cells is regulated by insulin. Impairment of glucose tolerance (i.e, longer time to clear given amount of glucose) indicates problems with maintenance of glucose homeostasis (insulin resistance, carbohydrate metabolism, diabetes, etc). According to the WHO, in a standard oral glucose tolerance test (OGTT), glucose level should be below 7.8 mmol/L (140 mg/dl) at 2 h. Levels between this and 11.1 mmol/L (200 mg/dl) indicate “impaired glucose tolerance”, and any level above 11.1 mmol/L (200 mg/dl) confirms a diagnosis of diabetes.
Materials and Reagents
- Mice (~20 C57BL/6J (B6) males of 2-3 months old)
- 70% ethanol
- Beta-D(+)-glucose (Sigma-Aldrich, catalog number: G8270 )
- NaCl
- KCl
- Sodium phosphate
- Phosphate buffered saline (PBS) (see Recipes)
Equipment
- ACCU-CHEK comfort curve glucometer (Roche Diagnostics, catalog number: 03537536001 ) (this product has been discontinued. Any new product of ACCU-CHEK should work fine as well)
Such device quantifies glucose amperometrically by measuring the current produced upon oxidation of glucose to gluconic acid by glucose oxidase, or to gluconolactone by dehydrogenase. - 27 gauge needle (Single-Use Needles, supplied by VWR, BD Medical, catalog number: BD305109 )
- Microvette CB300 Z serum separator (SARSTEDT, catalog number: 16.440.100 )
- Acrodisc 25 mm syringe filters w/ 0.2 μM HT Tuffryn membrane (Pall Corporation, catalog number: 4192 )
Procedure
Category
Biochemistry > Carbohydrate > Glucose
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