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Stereotaxic Injection of LPS or Your Reagents of Choice into Rat Substantia Nigra
Published: Nov 20, 2011 DOI: 10.21769/BioProtoc.154 Views: 12252
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Abstract
Stereotaxic injection is an invaluable tool for the creation of site-targeted lesions, injection of anatomical tracers, gene delivery by recombinant adeno-associated viruses and lentiviruses in mice and rats. Stereotaxic injection of LPS or 6-hydroxydopamine has been used to establish animal models of Parkinson’s disease, the most common neurodegenerative movement disorder. This protocol allows the investigation of central nervous system development and disease mechanisms. This protocol has been developed and improved over the years by various researchers in Dr. Hong’s lab, especially Dr. Bin Liu.
Materials and Reagents
- Two-month-old male F344 rats, body weight 220-250 g
- Nembutal
- Carprofen
- Betadine
- 70% ethanol
- Phosphate buffered saline (PBS)
- 4% paraformaldehyde
- Ocular lubricant (Puralube)
- LPS (Escherichia coli 0111: B4) (Sigma-Aldrich)
- Sterile normal saline (0.9%) or other vehicle for your reagents
- LPS stock solution (see Recipes)
Equipment
- Motorized microinjection pump
- Small-animal stereotaxic apparatus (rat stereotaxic apparatus)
- Sicroinjection apparatus
- Dental drill and #1 burrs
- Microknife
- Scalpel (#10)
- Tissue forceps
- Gauze
- Stereotaxic frame
- Autoclips/suture materials
Procedure
Category
Neuroscience > Nervous system disorders
Cell Biology > Tissue analysis > Tissue isolation
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