Protocol for Whole Cell Lysis of Yeast

Materials and Reagents

1. NaOH (Sigma-Aldrich)
2. Beta-mercaptoethanol (beta-ME) (Sigma-Aldrich)
3. 100% (w/v) trichloroacetic acid (TCA) (Sigma-Aldrich)
4. Pepstatin A (Sigma-Aldrich, catalog number: P5318 )
5. Leupeptin (Sigma-Aldrich, catalog number: L2884 )
6. PMSF (Sigma-Aldrich, catalog number: P7626 )
7. Acetone (Sigma-Aldrich)
8. Protease inhibitors
9. DMSO
10. Glycerol
11. BPB
12. Ethanol
13. SDS lysis buffer
14. 7.5 ml 1 M Tris-HCl (pH 6.8) (see Recipes)
15. DTT stock (see Recipes)
16. 5x SDS-PAGE gel loading dye (see Recipes)
17. Protease inhibitors (see Recipes)
    

Procedure