Phenol-chloroform Based RNA Extraction from Yeast

Materials and Reagents

1. DEPC treated water
2. Phenol (TE)/Chloroform (1:1)
3. 3 M NaAc (pH 5.2)
4. NaOAc
5. 10 mM EDTA
6. 10% SDS
7. EtOH
8. Hydroxyquinoline
9. Complete buffer A (see Recipes)
10. Buffer A phenol or RNA phenol (see Recipes)
11. TE phenol (see Recipes)
    

Equipment

1. RNase free tube
2. RNase-free plastic ware
3. Water bath
   

Procedure