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Chloroform-ethanol Isolation Genomic DNA from Mouse Tail
Published: Sep 20, 2011 DOI: 10.21769/BioProtoc.131 Views: 11849
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Abstract
This protocol describes a simple and fast method to purify genomic DNA from mouse tails using chloroform.
Materials and Reagents
- Proteinase K (20 mg/ml) (Life Technologies, Invitrogen™)
- KAcO (Sigma-Aldrich)
- Chloroform (Mallinckrodt)
- Ethanol (Pharmco-Aaper)
- Tris
- NaCl
- EDTA
- SDS
- Lysis buffer (see Recipes)
Equipment
- Centrifuges
- Incubator
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > DNA > DNA extraction
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