Expansion of Worms for Microarray, IP, ChIP and Similar Experiments

Materials and Reagents

1. Plates and culture materials:
   1. High growth (HG) plates
      
   2. Normal growth (NG) plates  
      
   3. OP50 or RNAi bacteria culture
      
   4. LB medium (autoclaved)
      Note: Prepare enough plates (with extra ones to replace contaminated plates).
      
   
   
2. Other materials:
   1. Antibiotics (carbenicillin, tetracycline)
      
   2. IPTG
      
   3. Cholesterol
      
   4. CaCl₂
      
   5. MgSO₄
      
   6. KPO₄
      
   7. KOH
      
   8. Sodium hypochlorite
      
   9. M9 buffer
      
   10. NP40 buffer
       
   11. O/N culture
       
   12. Triton X-100 (Sigma-Aldrich, catalog number: 9002-93-1 )
       
   13. Sodium hypochlorite (Thermo Fisher Scientific, catalog number: SS290-1 )
       
   14. 2x bleach solution (see Recipes)
       

Equipment

1. Beckman centrifuge and rotor (Beckman Coulter)
   
2. Low-temperature incubator (Thermo Fisher Scientific)
   
3. 15-ml conical tubes
   

Procedure