Improve Research Reproducibility A Bio-protocol resource
Peer-reviewed
tooltip

Extraction of Root Apoplastic Wall Fluid for Apoplastic Peroxidase Activity Assay

Xiyan Li Xiyan Li

Published: Sep 5, 2011 DOI: 10.21769/BioProtoc.127 Views: 16762

pdf PDF PDF
ask Ask a question
How to cite
Favorite
Cited by

Abstract

Plant roots secret a lot of peroxidases to counteract environmental influences. This protocol describes a way to extract root apoplastic wall fluid from Arabidopsis plants and to determine the peroxidase activity using guaiacol as substrate.

Keywords: Peroxidase search Root search Guaiacol search Apoplastic fluid search Enzyme activity search

Materials and Reagents

  1. 10 mM Na+-PO4 (pH 6.0)
  2. Guaiacol
  3. Agar
  4. MES-K
  5. 1/4x modified Hoagland medium (see general growth protocol for recipes)
  6. Solid medium (see Recipes)

Equipment

  1. UV-vis Spectrometer
  2. Arabidopsis growth facility
  3. 15 ml Falcon tube

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Plant Science > Plant cell biology > Cell structure

Biochemistry > Protein > Activity

Plant Science > Plant biochemistry > Protein > Isolation and purification

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
1 Q&A

How much of apoplastic fluid can be obtained per one root of 2.5 weeks old Arabidopsis plant?
edit 1 Answer 54 Views Jul 7, 2022