- Home
- Protocols
-
How to Use an Avestin Emulsiflex C3 Homogenizer to Disrupt Cells
Published: Jan 5, 2011 DOI: 10.21769/BioProtoc.11 Views: 23375
PDF 
Ask a question
Abstract
The EmulsiFlex-C3 homogenizer is powered by an electric motor. The pump does not require a compressor for it to run. This equipment can be used to disrupt cells at a large scale. The EmulsiFlex-C3 has a fixed flow-through capacity of 3 L/h. It has the ability to process samples as small as 10 ml. The homogenizing pressure is adjustable between 500 and 30,000 psi. In this protocol, we describe the use of the Avestin Emulsiflex C3 Homogenizer to disrupt S. pombe and S. cerevisiae cells.
Materials and Reagents
- S. pombe cells
- S. cerevisiae cells
- DI water
Equipment
- Avestin Emulsiflex C3 homogenizer (Avestin®)
Figure 1. Avestin Emulsiflex C3 homogenizer - Standard laboratory bench-top light microscope
Procedure
Category
Cell Biology > Cell viability > Cell lysis
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question
