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血浆中基因组DNA（gDNA）的快速提取和纯化

C Cytiva/思拓凡

Published: Mar 20, 2020 DOI: 10.21769/BioProtoc.1010591 Views: 3131

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该方案详细描述全血基因组DNA的提取和纯化解决方案。操作步骤简单，在提高产量和纯度的同时，减少RNA的干扰。Sera-Xtracta™基因组DNA（gDNA）提取纯化试剂盒可提供：1.高回收率：200μL血中可以提取4–8μg基因组DNA; 2. 高纯度：A260/A280数值大于1.7; 3. 简单快速：可在90分钟内完成基因组DNA的提取，且适用于自动化平台; 4. 易于使用：得到的高质量gDNA可以直接用于下游研究，包括克隆、酶切、PCR扩增、基因分型和二代测序(NGS)等。
关键词: cfDNA、游离DNA、液体活检、ctc、NIPT、高灵敏度、肿瘤诊断、产前筛查、核酸提取、精准医疗

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