产品说明书: KAPA Dual-Indexed Adapter Kit Illumina^® Platforms

Product Description

KAPA Dual-Indexed Adapter Kits for Illumina platforms are designed for use with KAPA DNA and RNA library preparation kits to construct libraries for sequencing on an Illumina sequencer. Each plate contains a set of 96 adapters, each with two, 8-nucleotide indexes (barcodes) for multiplexed sequencing applications.

The sequences of the sequencing indices (barcodes) included in KAPA Dual-Indexed Adapter Kits are given in Important Parameters: Index Sequences and Pooling Guidelines (p. 6). The highly dissimilar nature of indices minimizes the possibility of inaccurate index calling.

KAPA Adapter Dilution Buffer [10 mM Tris-HCl, (pH 8.0-8.5), 10 mM NaCl, 1 mM EDTA] is provided with the kit to ensure optimal performance when adapters require further dilution.

Product Applications

KAPA Dual-Indexed Adapters are used to uniquely label sequencing libraries generated from individual biological samples. This allows for the pooling of libraries prior to target capture or cluster generation, to enable multiplexed sequencing – which simplifies sample preparation and reduces the cost of next-generation sequencing for a wide range of applications.

Primary applications for the use of KAPA Dual-Indexed Adapter Kits for Illumina platforms include:
•Human whole genome sequencing, performed with an Illumina HiSeq X instrument
•whole exome or targeted sequencing, using Roche NimbleGen SeqCap EZ or IDT xGen Lockdown Probes or other hybridization capture systems, in combination with the appropriate blockers
•RNA-seq
•ChIP-seq
•other direct sequencing applications, e.g., microbial whole-genome sequencing on compatible platforms.

Note: KAPA Dual-Indexed Adapters are not methylated, and can therefore not be used for methyl-seq applications.