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Published: May 30, 2018 DOI: 10.21769/BioProtoc.1010125 Views: 12652
材料与试剂
仪器设备
实验步骤
溶液配方
Enzyme solution (终浓度) | 10 ml所需母液 |
0.6 M Mannitol | 3 ml 2 M Mannitol |
10 mM MES (pH 5.7) | 1 ml 0.1 M MES |
Cellulose RS (1.5%) | 0.15 g |
Macerozyme (0.75%) | 0.075 g |
0.1% BSA | 0.01 g |
1 mM CaCl2 | 10 µl 1 M CaCl2 |
ddH2O | 6 ml |
W5 solution终浓度 | 所需母液 |
154 mM NaCl | 10 ml (1.54 M NaCl) |
125 mM CaCl2 | 12.5 ml (1M CaCl2) |
5 mM KCl | 2.5 ml (0.2 M KCl) |
2 mM MES (pH 5.7) | 2 ml (0.1 M MES, pH 5.7) |
MMG solution终浓度 | 100 ml所需母液 |
0.6 M Mannitol | 30 ml 2 M Mannitol |
15 mM MgCl2 | 3 ml 0.5 M MgCl2 |
4 mM MES (pH 5.7) | 4 ml 0.1 M MES(pH 5.7) |
40% PEG终浓度 | 5 ml所需母液 |
40 % PEG4000 | 2g |
0.6 M Mannitol | 1.5 ml x 2 M Mannitol |
100 mM CaCl2 | 0.5 ml x 1 M CaCl2 |
Category
Plant Science > Plant cell biology > Cell isolation
Cell Biology > Cell isolation and culture > Transformation
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