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SDS-PAGE gel recipes
Last updated date: Apr 1, 2024 Views: 44900
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SDS-PAGE: enough for 2 x mini gels (Biorad system):
10 ml of 15% separating gel 4 ml of 6% stacking gel
2.4 ml H2O 2.7 ml H2O
5 ml acrylamide (30 %) 0.8 ml acrylamide (30 %)
2.5 ml 1.5 M tris (pH 8.8) 0.5 ml 1 M tris (pH 6.8)
50 µl SDS (20%) 20 µl SDS (20%)
100 µl APS (10%) 40 µl APS (10%)
10 µl TEMED 4 µl TEMED
10 ml of 13.5% separating gel 4 ml of 6% stacking gel
2.9 ml H2O 2.7 ml H2O
4.5 ml acrylamide (30 %) 0.8 ml acrylamide (30 %)
2.5 ml 1.5 M tris (pH 8.8) 0.5 ml 1 M tris (pH 6.8)
50 µl SDS (20%) 20 µl SDS (20%)
100 µl APS (10%) 40 µl APS (10%)
10 µl TEMED 4 µl TEMED
10 ml of 12% separating gel 4 ml of 6% stacking gel
3.4 ml H2O 2.7 ml H2O
4 ml acrylamide (30 %) 0.8 ml acrylamide (30 %)
2.5 ml 1.5 M tris (pH 8.8) 0.5 ml 1 M tris (pH 6.8)
50 µl SDS (20%) 20 µl SDS (20%)
100 µl APS (10%) 40 µl APS (10%)
10 µl TEMED 4 µl TEMED
10 ml of 10% separating gel 4 ml of 6% stacking gel
4.1 ml H2O 2.7 ml H2O
3.3 ml acrylamide (30 %) 0.8 ml acrylamide (30 %)
2.5 ml 1.5 M tris (pH 8.8) 0.5 ml 1 M tris (pH 6.8)
50 µl SDS (20%) 20 µl SDS (20%)
100 µl APS (10%) 40 µl APS (10%)
10 µl TEMED 4 µl TEMED
10 ml of 8% separating gel 4 ml of 6% stacking gel
4.7 ml H2O 2.7 ml H2O
2.7 ml acrylamide (30 %) 0.8 ml acrylamide (30 %)
2.5 ml 1.5 M tris (pH 8.8) 0.5 ml 1 M tris (pH 6.8)
50 µl SDS (20%) 20 µl SDS (20%)
100 µl APS (10%) 40 µl APS (10%)
10 µl TEMED 4 µl TEMED
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