IMMUNOFLUORESCENCE
Plate cells on Nun
Lab-Tek plates (coverslips)
Wash w/PBS
Fix with 4% PFA –
15 min
Wash w/PBS
Perm with 0.2%
Tritonx100 – 10 min
Wash w/PBS x3
Block w/ 10% NGS
with PBS – 30 min
Primary antibody for
1 hour RT or 4 degrees overnight
Wash w/PBS x3
Secondary antibody 1
hour RT
Wash w/PBS x3
Mount with
DAPI-Prolonged Gold