Fly Outcrossing

Improve Research Reproducibility A Bio-protocol resource

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Last updated date: Mar 20, 2024 Views: 1263

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Procedure

w^-; p[w⁺, transgene]/p[w⁺, transgene] ☿☿ x w^-; +/+ ♂♂ (w¹¹¹⁸ original)
↓ select red eye
w^-; p[w⁺, transgene]/+ ☿☿ x w^-; +/+ ♂♂ (w¹¹¹⁸ original)
↓ select red eye
w^-; p[w⁺, transgene]/+ ☿☿ x w^-; +/+ ♂♂ (w¹¹¹⁸ original)
↓ select red eye
w^-; p[w⁺, transgene]/+ ☿☿ x w^-; +/+ ♂♂ (w¹¹¹⁸ original)
↓ select red eye
w^-; p[w⁺, transgene]/+ ☿☿ x w^-; +/+ ♂♂ (w¹¹¹⁸ original)
↓ select red eye
w^-; p[w⁺, transgene]/+ ☿☿ x w^-; CyO/Sco or TM3, Sb/TM6B, Sb ♂♂ (w¹¹¹⁸ outcrossed)
↓
w^-; p[w⁺, transgene]/CyO or TM3, Sb ☿☿ x w^-; CyO/Sco or TM3, Sb/TM6B, Sb ♂♂ (w¹¹¹⁸ outcrossed)
↓
w^-; p[w⁺, transgene]/CyO or TM3, Sb ☿☿ x w^-; p[w⁺, transgene]/CyO or TM3, Sb ♂♂ (☿ and ♂ = same genotype)
↓
w^-; p[w⁺, transgene]/p[w⁺, transgene] ☿☿ x w^-; p[w⁺, transgene]/p[w⁺, transgene] ♂♂ (☿ and ♂ = same genotype)

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