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Materials and Reagents
| 试剂 | 分子式 | 分子量 | 品牌 | 货号 |
| EDTA, disodium salt, dihydrate | C10H14N2O8Na2·2H2O | 372.24 | BBI Life Sciences | EB0185 |
| Trizma base | C4H11NO3 | 121.14 | VETEC | V900483-500G |
| 冰醋酸 | C2H4O2 | 60.05 | 沪试 | 10000218 |
| 氢氧化钠（片状） | NaOH | 40.00 | 沪试 | 10019718 |
| miniQ水 | / | / | / | / |
Equipment
pH试纸；600ml、1500ml烧杯或烧瓶；量筒；搅拌器。
Procedure
配制EDTA储存液（以500ml，0.5M EDTA为例）
称取93.05gEDTA二钠盐
溶解在400ml去离子水中，用1M NaOH溶液调节pH至8.0使EDTA完全溶解
定容至500ml，保持溶液pH为8.0
配制50XTAE储存液
称量242gTris base
溶解在400ml去离子水中
加入57.1ml冰醋酸，100ml，0.5M EDTA（pH 8.0）
定容至1L
常温储存，(此时pH约为8.5)
配制TAE工作液（40mM Tris醋酸 1mM EDTA）
稀释50*TAE储存液至1*TAE工作液
Recipes
药品化学式 | 分子量 | 储存液浓度 | 配制1L需 | 配制2L需 | 配制4L需 | 配制5L需 |
Tris base | 121.14 | 1.998M | 242g | 484g | 968g | 1210g |
glacial acetic acid | 60.05 | 5.71% | 57.1ml | 114.2ml | 228.4ml | 285.5ml |
0.5M EDTA | 372.24 | 50mM | 100ml | 200ml | 400ml | 500ml |
References
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