Protocol for Quick DNA Preparation for Genomic Fly PCR

10 mM Tris-HCl (pH 8.2), 1 mM EDTA, 25 mM NaCl 

Proteinase K (10 mg/ml) 

Put fly into 1.5 ml tube and keep on ice. 

Crush with 50 ul of DNA extraction buffer using pipet tip 

Centrifuge for 1 min at maximum speed 

Transfer supernatant in PCR tube 

Incubate for 30 min at 37°C 

Incubate for 5 min at 95°C 

Puton ice immediately 

Store at -20°C