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Glucose tolerance test (GTT) and insulin tolerance test (ITT)

BV Bruno Vecchiatto
RS Rafael C. da Silva
TH Talita S. Higa
CM Cynthia R. Muller
AA Anna Laura V. Américo
VF Vanessa C. Fortunato-Lima
MF Marília M. Ferreira
LM Luiz Felipe Martucci
MF Miriam H. Fonseca-Alaniz
FE Fabiana S. Evangelista
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GTT and ITT were performed after the experimental protocol. Both experiments were performed in awake animals at 08:00 a.m. and after a 8-h fast. The glucose load (2 g/kg body weight) was injected as a bolus intraperitoneally, and the blood glucose levels were determined in caudal blood sampled at 0, 15, 30, 60, 90 and 120 min after glucose infusion. The glucose concentration was determined using a glucometer (AccuChek Advantage Roche Diagnostics®). After 72 h of GTT test, a similar procedure was performed for ITT. The insulin load (0.75 U/kg body weight) was injected as a bolus intraperitoneally, and the blood glucose levels were determined in caudal blood samples collected at 0, 5, 10, 15, 20, 25 and 30 min after injection. The values obtained between 5 and 30 min were used to calculate the rate constant for the disappearance of plasma glucose (kITT) according to the method proposed by Bonora et al. [21].

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