2.4.2.  Total antioxidant capacity assay

Asieh Karimani; Nasrin Ramezani; Amir Afkhami Goli; Mohammad Hossein Nazem Shirazi; Hosein Nourani; Amir Moghaddam Jafari

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2.4.2. Total antioxidant capacity assay

AK Asieh Karimani

NR Nasrin Ramezani

AG Amir Afkhami Goli

MS Mohammad Hossein Nazem Shirazi

HN Hosein Nourani

AJ Amir Moghaddam Jafari

This method is extracted from research article: Toxicol Rep, Jun 2021

Subchronic neurotoxicity of diazinon in albino mice: Impact of oxidative stress, AChE activity, and gene expression disturbances in the cerebral cortex and hippocampus on mood, spatial learning, and memory function

DOI: 10.1016/j.toxrep.2021.06.017

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Total antioxidant capacity was assayed in brain homogenates according to the ferric reducing antioxidant power (FRAP) assay. This method depends on the reduction of ferric tripyridyl-s-triazine (Fe³⁺-TPTZ) at low pH. Fe²⁺-TPTZ has an intensive blue color that can be monitored for up to 5 min at 593 nm by a spectrophotometer. Calculations were done by using a calibration curve of FeSO₄7H₂O (100−1000 mM) [2].

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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