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ELISA kit (501700, Cayman chemicals) was used to quantify the amount of 2′3′-cGAMP in cell lysates. The procedure was performed according to the manufacturer’s protocol. Additionally, cGAMP levels in primary fibroblasts were analyzed by means of LC–MS/MS with a 4000 QTrap (AB Sciex, Darmstadt, 3 Germany) and a Dionex UHPLC UltiMate 3000 (Thermo Fisher) or Prominence HPLC (Shimadzu, 4 Germany) similarly as was previously described for nucleotide quantification.
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