2.10. siRNA reverse transfection

Knockdown studies were performed using the reverse transfection method. Cells were seeded in a six‐well or 96‐well plate with lipid:siRNA mixture prepared using Lipofectamine 2000 (Invitrogen, Loughborough, UK) as per manufacturer's protocol. Final siRNA concentrations were 10 nmol. All dicer substrate short interfering RNAs against LINC00261 were purchased from Integrated DNA Technologies (Leuven, Belgium) [hs.Ri.LINC00261.13.1 (siRNA 1), hs.Ri.LINC00261.13.2 (siRNA 2), and hs.Ri.LINC00261.13.3 (siRNA 3)]. Control siRNAs were also purchased from IDT (negative control DS NC1 and positive control HPRT‐S1 DS). For murine cells, silencer select siRNAs against 9030622O22‐Rik were purchased (siRNA 1 = n404958, siRNA 2 = n440961, and siRNA 3 = n518634, as well as negative control; Thermo) and transfected with 25 nm in the same manner previously described. RNA was isolated using RNeasy Plus Mini Kit (Qiagen).