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Adjust the concentration of well-growing cells to 2×105 cells/ml. The culture medium of the treated RMS cells was taken out and washed with 1×PBS. The cells were stained with 1 mg/mL acridine orange and 1 mg/mL propidium iodide (Solarbio, China), and then incubated in the dark for 15 minutes. The stained cells were observed and photographed under a microscope.
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