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Naïve CD4+ T cells were enriched from PBMCs by negative selection using the EasySep Naïve CD4 T‐Cell Isolation Kit (StemCell Technologies). The purity of the naïve CD4+ T‐cell fraction, assessed by analysis of live CD45RA+CD27+ CD4+ T cells, was >90% for all the samples. Total T cells were enriched from PBMCs by negative selection using the EasySep Human T‐cell Enrichment Kit (StemCell Technologies).
Copyright and License information: ©2021 The Authors. published by Wiley‐VCH GmbH
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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