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The suprarenal aortas were dissected from sacrificed WT, β-arrestin-1–/– or β-arrestin-2–/– mice without the fixation by paraformaldehyde. The aortic tissues were cut into 5 mm-thick rings and cultured in DMEM for 48 h in the absence or presence of AngII (1 μM). The conditioned media were collected for ELISA measurement of MCP-1 and IL-6.
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