All data presented with error bars came from consist of at least three independent SCNT experiments (RNA‐seq analysis is exceptional and not repeated). Representative embryos were imaged for all strains, and images of the same embryo were displayed in the figures. The results are presented as the mean ± SEM. GraphPad Prism 5.0 software was used to calculate statistical significance with Student’s t test for relevant figures, as specified in the figure legends. Embryonic development was analysed by one‐way ANOVA with Duncan’s test using SAS software, while implantation and ESC‐derivation rates were analysed with the chi‐square test. P < .05 was regarded as statistically significant.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.