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MA Marine Angé
JP Julien De Poortere
AG Audrey Ginion
SB Sylvain Battault
MD Mélanie Dechamps
GM Giulio G. Muccioli
MR Martin Roumain
JM Johann Morelle
SD Sébastien Druart
TM Thomas Mathivet
LB Luc Bertrand
DC Diego Castanares-Zapatero
SH Sandrine Horman
CB Christophe Beauloye
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Tamoxifen (#T5648), LPS O55:B5 (#L2880), SBI0206965 (#SML1540), TMB substrate (#T040), Evans Blue (#E2129), and Transwell inserts (#3413) were from Sigma-Aldrich (Overijse, Belgium). Collagenase I (#17018029), anti-rat immunoglobulin G–coated magnetic beads (#11035), Cells-to-CT 1-Step TaqMan kit (#A25603), PRKAA1 FAM probe (#Mm01296696_m1), RPL32 VIC probe (Mm02528467_m1), siRNA negative control (#AM4635), siRNA PRKAA1 (#AM51334), and Lipofectamine RNAiMAX reagent (#13778-150) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). We also used Canagliflozin (#HY-10451, Medchem Express, Monmouth Junction, NJ, USA), and HRP-coupled streptavidin (#15:1000, DY998; R&D Systems, Minneapolis, MN, USA). The antibodies employed were rat anti-mouse platelet/endothelial cell adhesion molecule-1 (PECAM-1, #553370; BD Pharmingen) α1AMPK (#MA5-15815; Thermo Fisher Scientific), phospho-AMPK Thr172 (#2535; Cell Signaling Technology, Danvers, MA, USA), phospho-ACC S79 (#3661; Cell Signaling Technology), p38 MAPK (#9212; Cell Signaling Technology), phospho-p38 MAPK T180/Y182 (#9211; Cell Signaling Technology), heat shock protein of 27kDa (HSP-27) (#2402; Cell Signaling Technology), phospho-HSP27 S82 (#44534; Life Technologies, Thermo Fisher Scientific), eEF2 (#PA5-17794; Thermo Fisher Scientific), secondary horseradish peroxidase (HRP)-conjugated antibodies (#A0545; Sigma-Aldrich or #554002; BD Biosciences, San Jose, CA, USA), and Alexa Fluor-coupled secondary antibodies (#A21206; Invitrogen).

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